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imsuite.py

This module emulates MATLAB functions using our chosen library suite

bwareaopen = skimage.morphology.remove_small_objects module-attribute

Just skimage.morphology.remove_small_objects() see docs for more

bwlabel = scipy.ndimage.label module-attribute

just scipy.ndimage.label() see docs for more

histeq = skimage.exposure.equalize_hist module-attribute

Just skimage.exposure.equalize_hist() see docs for more

imbothat = skimage.morphology.black_tophat module-attribute

just skimage.morphology.black_tophat() see docs for more

imclose = skimage.morphology.closing module-attribute

just skimage.morphology.closing() see docs for more

imdilate = skimage.morphology.dilation module-attribute

Just skimage.morphology.dilation() see docs for more

imerode = skimage.morphology.erosion module-attribute

Just skimage.morphology.erosion() see docs for more

imfill = skimage.morphology.remove_small_holes module-attribute

Just skimage.morphology.remove_small_holes() see docs for more

imgaussfilt = scipy.ndimage.gaussian_filter module-attribute

just scipy.ndimage.gaussian_filter() see docs for more

imopen = skimage.morphology.opening module-attribute

just skimage.morphology.opening() see docs for more

impyramid_expand = skimage.transform.pyramid_expand module-attribute

just skimage.transform.pyramid_expand() see docs for more

impyramid_reduce = skimage.transform.pyramid_reduce module-attribute

just skimage.transform.pyramid_reduce() see docs for more

imreconstruct = skimage.morphology.reconstruction module-attribute

just skimage.morphology.reconstruction() see docs for more

imtophat = skimage.morphology.white_tophat module-attribute

just skimage.morphology.white_tophat() see docs for more

medfilt2 = skimage.filters.rank.median module-attribute

just skimage.filters.rank.median() see docs for more

regionprops = skimage.measure.regionprops module-attribute

just skimage.measure.regionprops() see docs for more

rgb2gray = skimage.color.rgb2gray module-attribute

Just skimage.color.rgb2gray() see docs for more

watershed = skimage.segmentation.watershed module-attribute

just skimage.segmentation.watershed() see docs for more

EdgeDetectionMethods

Bases: Enum

List of available edge detection methods

Source code in lavlab/imsuite.py
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class EdgeDetectionMethods(Enum):
    """List of available edge detection methods"""

    SOBEL = skimage.filters.sobel
    PREWITT = skimage.filters.prewitt
    ROBERTS = skimage.filters.roberts
    CANNY = skimage.feature.canny

apply_mask(img, mask)

Applies a binary mask to an image using PyVips.

Parameters:

Name Type Description Default
img ndarray

Image as a NumPy array.

required
mask ndarray

Binary mask as a NumPy array (same dimensions as img).

required

Returns:

Type Description
ndarray

Image with the mask applied.

Source code in lavlab/imsuite.py
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def apply_mask(
    img: Union[np.ndarray, pv.Image], mask: Union[np.ndarray, pv.Image]
) -> Union[np.ndarray, pv.Image]:
    """
    Applies a binary mask to an image using PyVips.

    Parameters
    ----------
    img : np.ndarray
        Image as a NumPy array.
    mask : np.ndarray
        Binary mask as a NumPy array (same dimensions as img).

    Returns
    -------
    np.ndarray
        Image with the mask applied.
    """
    if isinstance(img, np.ndarray):
        # Convert numpy array to pyvips Image
        vips_img = pv.Image.new_from_array(img)
    else:
        vips_img = img
    if isinstance(mask, np.ndarray):
        # Convert numpy array to pyvips Image
        vips_mask = pv.Image.new_from_array(mask)
    else:
        vips_mask = mask

    # Convert numpy arrays to pyvips images
    vips_img = pv.Image.new_from_array(img)
    vips_mask = pv.Image.new_from_array(mask)

    # Create a masked image using PyVips
    masked_img = vips_img * vips_mask

    # Convert the PyVips image back to a numpy array
    if isinstance(img, np.ndarray):
        return masked_img.numpy()
    return masked_img

dicomread(image_path, as_dataset=False)

Reads a dicom from a file.

Parameters:

Name Type Description Default
image_path PathLike or str

Path to a dicom file.

required

Returns:

Type Description
ndarray or Dataset

Array of pixel values, or Dataset if as_dataset=True.

Source code in lavlab/imsuite.py
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def dicomread(
    image_path: Union[os.PathLike, str], as_dataset=False
) -> Union[np.ndarray, pydicom.Dataset]:
    """Reads a dicom from a file.

    Parameters
    ----------
    image_path : os.PathLike or str
        Path to a dicom file.

    Returns
    -------
    np.ndarray or pydicom.Dataset
        Array of pixel values, or Dataset if as_dataset=True.
    """
    if as_dataset:
        return pydicom.dcmread(image_path)
    return pydicom.dcmread(image_path).pixel_array

dicomread_volume(dicom_dir, as_sequence=False)

Reads a dicom series from a directory.

Parameters:

Name Type Description Default
dicom_dir PathLike or str or list[BytesIO or PathLike or str]

Path to directory with the dicoms or list of dicoms as path or bytes.

required
as_sequence bool

If True, returns a pydicom sequence of pydicom datasets, by default False.

False

Returns:

Type Description
ndarray or Sequence

Dicom series as numpy volume, or Sequence if as_sequence=True.

Source code in lavlab/imsuite.py
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def dicomread_volume(
    dicom_dir: Union[os.PathLike, str, list[Union[io.BytesIO, os.PathLike, str]]],
    as_sequence=False,
) -> Union[np.ndarray, pydicom.Sequence]:
    """Reads a dicom series from a directory.

    Parameters
    ----------
    dicom_dir : os.PathLike or str or list[io.BytesIO or os.PathLike or str]
        Path to directory with the dicoms or list of dicoms as path or bytes.
    as_sequence : bool, optional
        If True, returns a pydicom sequence of pydicom datasets, by default False.

    Returns
    -------
    np.ndarray or pydicom.Sequence
        Dicom series as numpy volume, or Sequence if as_sequence=True.
    """

    # Get a list of all DICOM files in the directory
    if isinstance(dicom_dir, list):
        dicom_files = dicom_dir
    else:
        dicom_files = [
            os.path.join(dicom_dir, filename)
            for filename in os.listdir(dicom_dir)
            if filename.endswith(".dcm")
        ]
        if not dicom_files:
            dicom_files = [
                os.path.join(dicom_dir, filename) for filename in os.listdir(dicom_dir)
            ]

    dicoms = []
    for file in dicom_files:
        try:
            dicoms.append(pydicom.dcmread(file))
        except InvalidDicomError:
            LOGGER.warning(f"Invalid DICOM file: {file}")

    if len(dicoms) == 0:
        raise ValueError("No valid DICOM files found in the directory.")
    if len({ds.SeriesInstanceUID for ds in dicoms}) != 1:
        raise ValueError("All DICOM files must belong to the same series.")

    dicoms.sort(key=lambda x: x.InstanceNumber)
    if as_sequence:
        return pydicom.Sequence(dicoms)

    # Read the first DICOM file to get image dimensions
    first_ds = dicoms[0]
    rows = int(first_ds.Rows)
    columns = int(first_ds.Columns)
    slices = len(dicoms)

    # Initialize a 3D array to store pixel data
    volume = np.zeros((rows, columns, slices), dtype=np.uint16)

    # Read each DICOM file and store pixel data in the volume array
    for i, ds in enumerate(dicoms):
        volume[:, :, i] = ds.pixel_array
    return volume

dicomseg_to_nifti_vol(dicom_seg_ds)

Converts a DICOM Segmentation object to a 3D numpy array.

Parameters:

Name Type Description Default
dicom_seg_ds Segmentation

DICOM Segmentation object.

required

Returns:

Type Description
ndarray

3D numpy array of the segmentation.

Source code in lavlab/imsuite.py
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def dicomseg_to_nifti_vol(dicom_seg_ds: hd.seg.Segmentation) -> np.ndarray:
    """
    Converts a DICOM Segmentation object to a 3D numpy array.

    Parameters
    ----------
    dicom_seg_ds : hd.seg.Segmentation
        DICOM Segmentation object.

    Returns
    -------
    np.ndarray
        3D numpy array of the segmentation.
    """
    rows, cols = dicom_seg_ds.Rows, dicom_seg_ds.Columns
    slice_count = len(
        dicom_seg_ds.ReferencedSeriesSequence[0].ReferencedInstanceSequence
    )
    full_dims = (slice_count, rows, cols)
    full_vol = np.zeros(full_dims)

    for i, frame in enumerate(dicom_seg_ds.PerFrameFunctionalGroupsSequence):
        slice_idx = frame.FrameContentSequence[0].DimensionIndexValues[1]
        full_vol[slice_idx] = dicom_seg_ds.pixel_array[i]
    # lps to ras
    full_vol = np.flip(full_vol, axis=0)
    full_vol = np.flip(full_vol, axis=1)
    full_vol = full_vol.transpose((2, 1, 0))
    return full_vol

dicomsegread(image_path, as_volume)

Reads a dicom segmentation from a file.

Parameters:

Name Type Description Default
image_path PathLike or str

Path to a dicom segmentation file.

required

Returns:

Type Description
ndarray

Array of pixel values.

Source code in lavlab/imsuite.py
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def dicomsegread(
    image_path: Union[os.PathLike, str], as_volume: True
) -> Union[np.ndarray, hd.seg.Segmentation]:
    """Reads a dicom segmentation from a file.

    Parameters
    ----------
    image_path : os.PathLike or str
        Path to a dicom segmentation file.

    Returns
    -------
    np.ndarray
        Array of pixel values.
    """
    dicom_seg = hd.seg.segread(image_path)
    if as_volume:
        return dicomseg_to_nifti_vol(dicom_seg)
    return dicom_seg.pixel_array

dicomwrite(img, path, write_like_original=False)

Writes an image to path. kwargs are passthrough to wrapped function.

Parameters:

Name Type Description Default
img ndarray or Dataset

Numpy array or Dicom dataset. If array, Dicom dataset is created.

required
path PathLike or str

Path to desired file.

required
kwargs dict

Additional arguments to pass to pydicom.dcmwrite.

required

Returns:

Type Description
str

Path of newly created file.

Source code in lavlab/imsuite.py
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def dicomwrite(
    img: Union[np.ndarray, pydicom.Dataset],
    path: Union[os.PathLike, str],
    write_like_original: bool = False,
) -> str:
    """Writes an image to path. kwargs are passthrough to wrapped function.

    Parameters
    ----------
    img : np.ndarray or pydicom.Dataset
        Numpy array or Dicom dataset. If array, Dicom dataset is created.
    path : os.PathLike or str
        Path to desired file.
    kwargs : dict
        Additional arguments to pass to pydicom.dcmwrite.

    Returns
    -------
    str
        Path of newly created file.
    """
    path = str(path)
    if not path.endswith(".dcm"):
        LOGGER.warning(
            "Dicom extension not detected in path! Dicoms should end in .dcm! Appending .dcm..."
        )
        path += ".dcm"
    if isinstance(img, np.ndarray):
        LOGGER.warning(
            "Chances are you won't be adding all the metadata you want and need by passing a numpy array to dicomwrite! Use pydicom.Dataset instead! Converting to a Dataset and continuing"  # pylint: disable=line-too-long
        )
        img = pydicom.Dataset()
        img.PixelData = img.tobytes()
        img.Rows, img.Columns = img.shape
    pydicom.dcmwrite(path, img, write_like_original)
    return path

draw_shapes(img, shape_points)

Draws a list of shape points onto the input image using PyVips with SVG xml.

Warnings

No safety checks! Make sure img and shape_points are for the same downsample factor!

Parameters:

Name Type Description Default
img Union[ndarray, Image]

Input image as a NumPy array or pyvips Image.

required
shape_points List[Tuple[int, Tuple[int, int, int], List[Tuple[int, int]]]]

List of tuples containing shape ID, RGB color, and list of points. Expected to use output from lavlab.omero_util.getShapesAsPoints.

required

Returns:

Type Description
Union[ndarray, Image]

Modified image with shapes drawn, same type as input.

Source code in lavlab/imsuite.py
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def draw_shapes(
    img: Union[np.ndarray, pv.Image],
    shape_points: list[tuple[int, tuple[int, int, int], list[tuple[int, int]]]],
) -> Union[np.ndarray, pv.Image]:
    """
    Draws a list of shape points onto the input image using PyVips with SVG xml.

    Warnings
    --------
       No safety checks! Make sure img and shape_points are for the same downsample factor!

    Parameters
    ----------
    img : Union[np.ndarray, pyvips.Image]
        Input image as a NumPy array or pyvips Image.
    shape_points : List[Tuple[int, Tuple[int, int, int], List[Tuple[int, int]]]]
        List of tuples containing shape ID, RGB color, and list of points.
        Expected to use output from lavlab.omero_util.getShapesAsPoints.

    Returns
    -------
    Union[np.ndarray, pyvips.Image]
        Modified image with shapes drawn, same type as input.
    """
    if isinstance(img, np.ndarray):
        # Convert numpy array to pyvips Image
        pv_img = pv.Image.new_from_array(img)
    else:
        pv_img = img

    width = pv_img.width
    height = pv_img.height
    svg_header = (
        f'<svg viewBox="0 0 {width} {height}" '
        f'xmlns="http://www.w3.org/2000/svg" '
        f'shape-rendering="crispEdges">'
    )
    svg_shapes = ""

    for _, rgb, xy in shape_points:
        hex_color = f"#{rgb[0]:02x}{rgb[1]:02x}{rgb[2]:02x}"
        points = " ".join([f"{x},{y}" for y, x in xy])
        svg_shapes += f'<polygon points="{points}" fill="{hex_color}"/>'

    svg_footer = "</svg>"
    svg = svg_header + svg_shapes + svg_footer
    svg_img = pv.Image.svgload_buffer(svg.encode("utf-8"))

    # Composite the SVG image over the original image
    composite_img = pv_img.composite2(svg_img, "over", x=0, y=0)

    # Return the modified image in the same type as the input
    if isinstance(img, np.ndarray):
        return composite_img.numpy()
    return composite_img

edge(img_arr, method='SOBEL', **kwargs)

Edge detection.

Parameters:

Name Type Description Default
img_arr ndarray

Input image as a numpy array.

required
method str

One of the enumerated methods: SOBEL, PREWITT, ROBERTS, CANNY. Defaults to SOBEL.

'SOBEL'

Returns:

Type Description
ndarray

Edge map.

Source code in lavlab/imsuite.py
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def edge(img_arr: np.ndarray, method: str = "SOBEL", **kwargs) -> np.ndarray:
    """Edge detection.

    Parameters
    ----------
    img_arr : np.ndarray
        Input image as a numpy array.
    method : str, optional
        One of the enumerated methods: SOBEL, PREWITT, ROBERTS, CANNY. Defaults to SOBEL.

    Returns
    -------
    np.ndarray
        Edge map.
    """
    function = getattr(EdgeDetectionMethods, method, None)
    if function is None:
        raise KeyError(f"{method} is not a valid edge detection method!")
    return function(img_arr, **kwargs)

get_color_region_contours(img_arr, rgb_val, axis=-1)

Finds the contours of all areas with a given rgb value. Useful for finding drawn ROIs.

Parameters:

Name Type Description Default
img_arr ndarray

Image with ROIs. Converts PIL Image to np array for processing.

required
rgb_val tuple[int, int, int]

Red, Green, and Blue values for the roi color.

required
axis

Which axis is the color channel. Default is the last axis [:,:,color]

-1

Returns:

Type Description
list[tuple[int(None), rgb_val, contours]]

Returns list of lavlab shapes.

Source code in lavlab/imsuite.py
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def get_color_region_contours(
    img_arr: np.ndarray, rgb_val: tuple[int, int, int], axis=-1
) -> np.ndarray:
    """
    Finds the contours of all areas with a given rgb value. Useful for finding drawn ROIs.

    Parameters
    ----------
    img_arr: np.ndarray or PIL.Image
        Image with ROIs. Converts PIL Image to np array for processing.
    rgb_val: tuple[int,int,int]
        Red, Green, and Blue values for the roi color.
    axis: int, Default: -1
        Which axis is the color channel. Default is the last axis [:,:,color]

    Returns
    -------
    list[ tuple[int(None), rgb_val, contours] ]
        Returns list of lavlab shapes.
    """
    assert isinstance(img_arr, np.ndarray)
    mask_bin = np.all(img_arr == rgb_val, axis=axis)
    contours = skimage.measure.find_contours(mask_bin, level=0.5)
    del mask_bin
    # wrap in lavlab shape convention
    for i, contour in enumerate(contours):
        contour = [(x, y) for y, x in contour]
        contours[i] = (None, rgb_val, contour)
    return contours

get_downsample_from_dimensions(base_shape, sample_shape)

Essentially an alias for np.divide().

Finds the ratio between a base array shape and a sample array shape by dividing each axis.

Parameters:

Name Type Description Default
base_shape tuple[int, ...]

Shape of the larger image. (Image.size / base_nparray.shape)

required
sample_shape tuple[int, ...]

Shape of the smaller image. (Image.size / sample_nparray.shape)

required

Raises:

Type Description
AssertionError

Asserts that the input shapes have the same amount of axes

Returns:

Type Description
tuple(int) * x

Returns a tuple containing the downsample factor of each axis for the sample array.

Source code in lavlab/imsuite.py
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def get_downsample_from_dimensions(
    base_shape: tuple[int, ...], sample_shape: tuple[int, ...]
) -> tuple[float, ...]:
    """
    Essentially an alias for np.divide().

    Finds the ratio between a base array shape and a sample array shape by dividing each axis.

    Parameters
    ----------
    base_shape: tuple(int)*x
        Shape of the larger image. (Image.size / base_nparray.shape)
    sample_shape: tuple(int)*x
        Shape of the smaller image. (Image.size / sample_nparray.shape)

    Raises
    ------
    AssertionError
        Asserts that the input shapes have the same amount of axes

    Returns
    -------
    tuple(int)*x
        Returns a tuple containing the downsample factor of each axis for the sample array.

    """
    assert len(base_shape) == len(sample_shape)
    return tuple(np.divide(base_shape, sample_shape))

imadjust(img_arr, tol=1, vin=(0, 255), vout=(0, 255))

Matlab's imadjust in Python.

Parameters:

Name Type Description Default
img_arr ndarray

Grayscale image.

required
tol int

Tolerance, from 0 to 100, defaults to 1.

1
vin tuple[int, int]

Input image bounds, defaults to (0,255).

(0, 255)
vout tuple[int, int]

Output image bounds, defaults to (0,255).

(0, 255)

Returns:

Type Description
ndarray

Intensity adjusted image.

Source code in lavlab/imsuite.py
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def imadjust(
    img_arr: np.ndarray,
    tol: int = 1,
    vin: tuple[int, int] = (0, 255),
    vout: tuple[int, int] = (0, 255),
) -> np.ndarray:
    """
    Matlab's imadjust in Python.

    Parameters
    ----------
    img_arr : np.ndarray
        Grayscale image.
    tol : int, optional
        Tolerance, from 0 to 100, defaults to 1.
    vin : tuple[int, int], optional
        Input image bounds, defaults to (0,255).
    vout : tuple[int, int], optional
        Output image bounds, defaults to (0,255).

    Returns
    -------
    np.ndarray
        Intensity adjusted image.
    """
    assert len(img_arr.shape) == 2, "Input image should be 2-dims"

    tol = max(0, min(100, tol))

    if tol > 0:
        # Compute in and out limits
        hist = np.histogram(img_arr, bins=256, range=(0, 255))[0]

        # Cumulative histogram
        cum = hist.cumsum()

        # Compute bounds
        total = img_arr.size
        low_bound = total * tol / 100
        upp_bound = total * (100 - tol) / 100
        vin = (bisect.bisect_left(cum, low_bound), bisect.bisect_left(cum, upp_bound))

    # Avoid division by zero by setting vin[1] if it's the same as vin[0]
    if vin[0] == vin[1]:
        vin = (vin[0], vin[0] + 1)

    # Stretching
    scale = (vout[1] - vout[0]) / (vin[1] - vin[0])
    vs = img_arr - vin[0]
    vs[img_arr < vin[0]] = 0
    vd = vs * scale + 0.5 + vout[0]
    vd[vd > vout[1]] = vout[1]
    dst = vd

    return dst.astype(np.uint8)

imbinarize(image)

Generates a binary image from a grayscale image using Otsu's thresholding.

Parameters:

Name Type Description Default
image ndarray

numpy array

required

Returns:

Type Description
ndarray

binary image

Source code in lavlab/imsuite.py
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def imbinarize(image):
    """
    Generates a binary image from a grayscale image using Otsu's thresholding.

    Parameters
    ----------
    image : np.ndarray
        numpy array

    Returns
    -------
    np.ndarray
        binary image
    """
    if len(image.shape) == 3:
        image = rgb2gray(image)

    # Apply Otsu's thresholding
    thresh = skimage.filters.threshold_otsu(image)
    binary_image = image > thresh
    return binary_image

imcomplement(img_arr)

Generates the image's complement (inverts the image).

Parameters:

Name Type Description Default
img_arr ndarray

Input image.

required

Returns:

Type Description
ndarray

Inverted image.

Source code in lavlab/imsuite.py
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def imcomplement(img_arr: np.ndarray) -> np.ndarray:
    """Generates the image's complement (inverts the image).

    Parameters
    ----------
    img_arr : np.ndarray
        Input image.

    Returns
    -------
    np.ndarray
        Inverted image.
    """
    return ~img_arr

imcrop(img_arr, dims)

Crops a region from an image using pyvips

Parameters:

Name Type Description Default
img_arr Union[ndarray, Image]

Input image as a NumPy array or pyvips Image.

required
dims tuple[int, int, int, int]

Desired dimensions: left, top, width, height.

required

Returns:

Type Description
Union[ndarray, Image]

Cropped image.

Source code in lavlab/imsuite.py
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def imcrop(
    img_arr: Union[np.ndarray, pv.Image], dims: tuple[int, int, int, int]
) -> Union[np.ndarray, pv.Image]:
    """Crops a region from an image using pyvips

    Parameters
    ----------
    img_arr : Union[np.ndarray, pv.Image]
        Input image as a NumPy array or pyvips Image.
    dims : tuple[int,int,int,int]
        Desired dimensions: left, top, width, height.

    Returns
    -------
    Union[np.ndarray, pv.Image]
        Cropped image.
    """
    img = img_arr
    if not isinstance(img, pv.Image):
        assert isinstance(img, np.ndarray)
        img_arr = pv.Image.new_from_array(img)
    assert isinstance(img_arr, pv.Image)

    rotated_img = img_arr.crop(*dims)
    assert isinstance(rotated_img, pv.Image)

    # Retain the input type in the output
    if isinstance(img, np.ndarray):
        return rotated_img.numpy()
    return rotated_img

imhist(img_arr, bins=256)

Plots and displays histogram of image intensity values

Parameters:

Name Type Description Default
img_arr ndarray

Input image.

required
bins int

Number of bins, defaults to 256.

256

Returns:

Type Description
None
Source code in lavlab/imsuite.py
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def imhist(img_arr: np.ndarray, bins=256) -> None:
    """Plots and displays histogram of image intensity values

    Parameters
    ----------
    img_arr : np.ndarray
        Input image.
    bins : int, optional
        Number of bins, defaults to 256.

    Returns
    -------
    None
    """
    # Calculate the histogram
    matplotlib.pyplot.hist(img_arr.ravel(), bins=bins, range=(0.0, 256.0))

    # Set the title and labels
    matplotlib.pyplot.title("Histogram of Image Intensity Values")
    matplotlib.pyplot.xlabel("Intensity Value")
    matplotlib.pyplot.ylabel("Frequency")

    # Show the histogram
    matplotlib.pyplot.show()

imread(image_path, wild=False)

Loads an image from a file.

Parameters:

Name Type Description Default
image_path Union[PathLike, BinaryIO]

Path to image.

required
wild bool

If True, will not warn about niche formats, by default False.

False

Returns:

Type Description
ndarray

Array of pixel values.

Warnings

This function can cause an OOM error if the image is too large! Use wsiread if your image is large.

Source code in lavlab/imsuite.py
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def imread(image_path: Union[os.PathLike, BinaryIO, str], wild=False) -> np.ndarray:
    """
    Loads an image from a file.

    Parameters
    ----------
    image_path : Union[os.PathLike, BinaryIO]
        Path to image.
    wild : bool, optional
        If True, will not warn about niche formats, by default False.

    Returns
    -------
    np.ndarray
        Array of pixel values.

    Warnings
    --------
    This function can cause an OOM error if the image is too large!
    Use wsiread if your image is large.
    """
    if isinstance(image_path, BinaryIO):
        return pv.Image.new_from_buffer(image_path, "").numpy()
    image_path = str(image_path)
    if image_path.endswith(".nii") or image_path.endswith(".nii.gz"):
        if not wild:
            LOGGER.warning(
                "Nifti detected, use niftiread() for clarity if possible otherwise enable wild. Using niftiread..."  # pylint: disable=line-too-long
            )
        nii = niftiread(image_path)
        assert isinstance(nii, np.ndarray)
        return nii
    if image_path.endswith(".dcm"):
        if not wild:
            LOGGER.warning(
                "Dicom detected, use dicomread() for clarity if possible otherwise enable wild. Using dicomread..."  # pylint: disable=line-too-long
            )
        dcm = dicomread(image_path)
        assert isinstance(dcm, np.ndarray)
        return dcm
    if os.path.isdir(image_path):
        if not wild:
            LOGGER.warning(
                "Dicom directory detected, use dicomread_volume() for clarity if possible otherwise enable wild. Using dicomread_volume"  # pylint: disable=line-too-long
            )
        dcm_vol = dicomread_volume(image_path)
        assert isinstance(dcm_vol, np.ndarray)
        return dcm_vol

    img = pv.Image.new_from_file(str(image_path))
    assert isinstance(img, pv.Image)
    if not is_memsafe_pvimg(img):
        LOGGER.warning("Image is too large for memory! Use wsiread() for large images.")
        return wsiread(image_path)
    return img.numpy()

imresize(img, factor)

Convenience wrapper for resize functions. Uses pyvips for 2D and skimage for 3D.

Parameters:

Name Type Description Default
img Union[ndarray, Image]

Input image as a NumPy array or pyvips Image.

required
factor Union[int, float, Tuple[int, ...]]

Scale factor (if int or float) or desired dimensions (if tuple).

required

Returns:

Type Description
Union[ndarray, Image]

Resized image as a NumPy array or pyvips Image.

Source code in lavlab/imsuite.py
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def imresize(
    img: Union[np.ndarray, pv.Image], factor: Union[int, float, tuple[int, ...]]
) -> Union[np.ndarray, pv.Image]:
    """
    Convenience wrapper for resize functions. Uses pyvips for 2D and skimage for 3D.

    Parameters
    ----------
    img : Union[np.ndarray, pv.Image]
        Input image as a NumPy array or pyvips Image.
    factor : Union[int, float, Tuple[int, ...]]
        Scale factor (if int or float) or desired dimensions (if tuple).

    Returns
    -------
    Union[np.ndarray, pv.Image]
        Resized image as a NumPy array or pyvips Image.
    """
    if isinstance(img, np.ndarray):
        dimensions = len(img.shape)
        if dimensions == 3 and img.shape[2] == 3:
            dimensions = 2
            width, height = img.shape[1], img.shape[0]
        elif dimensions == 2:
            height, width = img.shape
    elif isinstance(img, pv.Image):
        dimensions = 2
        width, height = img.width, img.height
    else:
        raise TypeError(
            "Unsupported image type. Only np.ndarray and pyvips.Image are supported."
        )

    if dimensions == 2:
        if isinstance(factor, (int, float)):
            factor_tuple = (float(factor), float(factor))
        elif isinstance(factor, tuple) and len(factor) == 2:
            factor_tuple = (factor[1] / width, factor[0] / height)

        if isinstance(img, np.ndarray):
            pv_img = pv.Image.new_from_array(img)
            return imresize2d(pv_img, factor_tuple).numpy()
        return imresize2d(img, factor_tuple)
    if dimensions == 3:
        assert isinstance(factor, tuple)
        return imresize3d(img, (factor[0], factor[1], factor[2]))
    raise ValueError(
        "Unsupported image dimensions. Only 2D and 3D images are supported."
    )

imresize2d(img, scale)

Resizes a 2D image using pyvips' resize function.

Parameters:

Name Type Description Default
img Image

Input 2D image.

required
target_size Tuple[int, int]

Scale factor h and v.

required

Returns:

Type Description
Image

Resized image as a pyvips Image.

Source code in lavlab/imsuite.py
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def imresize2d(img: pv.Image, scale: tuple[float, float]) -> pv.Image:
    """
    Resizes a 2D image using pyvips' resize function.

    Parameters
    ----------
    img : pv.Image
        Input 2D image.
    target_size : Tuple[int, int]
        Scale factor h and v.

    Returns
    -------
    pv.Image
        Resized image as a pyvips Image.
    """
    return img.resize(scale[0], vscale=scale[1])

imresize3d(img, target_size)

Resizes a 3D image using skimage's resize function.

Parameters:

Name Type Description Default
img ndarray

Input 3D image.

required
target_size Tuple[int, int, int]

Desired dimensions as a tuple of (depth, height, width).

required

Returns:

Type Description
ndarray

Resized image as a NumPy array.

Source code in lavlab/imsuite.py
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def imresize3d(img: np.ndarray, target_size: tuple[int, int, int]) -> np.ndarray:
    """
    Resizes a 3D image using skimage's resize function.

    Parameters
    ----------
    img : np.ndarray
        Input 3D image.
    target_size : Tuple[int, int, int]
        Desired dimensions as a tuple of (depth, height, width).

    Returns
    -------
    np.ndarray
        Resized image as a NumPy array.
    """
    return skimage.transform.resize(img, target_size)

imrotate(img_arr, degrees)

Rotates an input image using pyvips.

Parameters:

Name Type Description Default
img_arr Union[ndarray, Image]

Input image as a NumPy array or pyvips Image.

required
degrees int or float

Rotation in degrees.

required

Returns:

Type Description
Union[ndarray, Image]

Rotated image.

Source code in lavlab/imsuite.py
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def imrotate(
    img_arr: Union[np.ndarray, pv.Image], degrees: Union[int, float]
) -> Union[np.ndarray, pv.Image]:
    """Rotates an input image using pyvips.

    Parameters
    ----------
    img_arr : Union[np.ndarray, pv.Image]
        Input image as a NumPy array or pyvips Image.
    degrees : int or float
        Rotation in degrees.

    Returns
    -------
    Union[np.ndarray, pv.Image]
        Rotated image.
    """
    img = img_arr
    if not isinstance(img, pv.Image):
        assert isinstance(img, np.ndarray)
        img_arr = pv.Image.new_from_array(img)
    assert isinstance(img_arr, pv.Image)

    rotated_img = img_arr.rotate(degrees, interpolate=pv.Interpolate.new("nearest"))
    assert isinstance(rotated_img, pv.Image)

    # Retain the input type in the output
    if isinstance(img, np.ndarray):
        return rotated_img.numpy()
    return rotated_img

imshow(image, **kwargs)

Just matplotlib.pyplot.imshow() see docs for more

Source code in lavlab/imsuite.py
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def imshow(image, **kwargs):
    """Just matplotlib.pyplot.imshow() see docs for more"""
    matplotlib.pyplot.figure()
    matplotlib.pyplot.imshow(image, **kwargs)

imwarp(img_arr, affine_matrix)

Affine warps a region from an image using pyvips

Parameters:

Name Type Description Default
img_arr Union[ndarray, Image]

Input image as a NumPy array or pyvips Image.

required
affine tuple[float, float, float, float]

4 element affine transform matrix

required

Returns:

Type Description
Union[ndarray, Image]

Warped image

Source code in lavlab/imsuite.py
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def imwarp(
    img_arr: Union[np.ndarray, pv.Image],
    affine_matrix: tuple[float, float, float, float],
) -> Union[np.ndarray, pv.Image]:
    """Affine warps a region from an image using pyvips

    Parameters
    ----------
    img_arr : Union[np.ndarray, pv.Image]
        Input image as a NumPy array or pyvips Image.
    affine : tuple[float,float,float,float]
        4 element affine transform matrix

    Returns
    -------
    Union[np.ndarray, pv.Image]
        Warped image
    """
    img = img_arr
    if not isinstance(img, pv.Image):
        assert isinstance(img, np.ndarray)
        img_arr = pv.Image.new_from_array(img)
    assert isinstance(img_arr, pv.Image)

    rotated_img = img_arr.affine(
        affine_matrix, interpolate=pv.Interpolate.new("nearest")
    )
    assert isinstance(rotated_img, pv.Image)

    # Retain the input type in the output
    if isinstance(img, np.ndarray):
        return rotated_img.numpy()
    return rotated_img

imwrite(img, path, **kwargs)

Writes an image to path. kwargs are passthrough to wrapped function.

Parameters:

Name Type Description Default
img Union[ndarray, Image]

Numpy array or PyVips image.

required
path PathLike or str

Path to desired file.

required

Returns:

Type Description
str

Path of newly created file.

Source code in lavlab/imsuite.py
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def imwrite(
    img: Union[np.ndarray, pv.Image], path: Union[os.PathLike, str], **kwargs
) -> str:
    """Writes an image to path. kwargs are passthrough to wrapped function.

    Parameters
    ----------
    img : Union[np.ndarray, pv.Image]
        Numpy array or PyVips image.
    path : os.PathLike or str
        Path to desired file.

    Returns
    -------
    str
        Path of newly created file.
    """
    path = str(path)
    if path.endswith(".nii") or path.endswith(".nii.gz"):
        LOGGER.warning(
            "Nifti detected, use niftiwrite() for clarity! Using niftiwrite..."
        )
        return niftiwrite(img, path, **kwargs)
    if path.endswith(".dcm"):
        LOGGER.warning(
            "Dicom detected, use dicomwrite() for clarity! Using dicomwrite..."
        )
        return dicomwrite(img, path, **kwargs)
    if not isinstance(img, pv.Image):
        assert isinstance(img, np.ndarray)
        img = pv.Image.new_from_array(img)
    assert isinstance(img, pv.Image)
    img.write_to_file(path, **kwargs)
    return path

niftiread(image_path, as_nib=False)

Loads a nifti from a file.

Parameters:

Name Type Description Default
image_path PathLike or str

Path to nifti.

required
as_nib bool

If True, returns a nibabel image class, by default False.

False

Returns:

Type Description
ndarray or FileBasedImage

Array of pixel values or appropriate Nifti image class when as_nib=True.

Source code in lavlab/imsuite.py
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def niftiread(
    image_path: Union[os.PathLike, str, io.BytesIO], as_nib=False
) -> Union[np.ndarray, FileBasedImage]:
    """Loads a nifti from a file.

    Parameters
    ----------
    image_path : os.PathLike or str
        Path to nifti.
    as_nib : bool, optional
        If True, returns a nibabel image class, by default False.

    Returns
    -------
    np.ndarray or FileBasedImage
        Array of pixel values or appropriate Nifti image class when as_nib=True.
    """
    if isinstance(image_path, io.BytesIO):
        return nib.Nifti1Image.from_stream(image_path)
    image_path = str(image_path)
    if as_nib:
        return nib.load(image_path)  # type: ignore
    return nib.load(image_path).get_fdata()  # type: ignore

niftiwrite(img, path, affine=None, **kwargs)

Writes an image to path. kwargs are passthrough to wrapped function.

Parameters:

Name Type Description Default
img ndarray or Nifti1Image or Nifti2Image

Numpy array or Nifti image. If array, Nifti image is created.

required
path PathLike or str

Path to desired file.

required
affine ndarray

Affine matrix for the image, by default uses nibabel's default.

None
kwargs dict

Additional arguments to pass to nib.save.

{}

Returns:

Type Description
str

Path of newly created file.

Source code in lavlab/imsuite.py
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def niftiwrite(
    img: Union[np.ndarray, nib.Nifti1Image, nib.Nifti2Image],
    path: Union[os.PathLike, str],
    affine=None,
    **kwargs,
) -> str:
    """Writes an image to path. kwargs are passthrough to wrapped function.

    Parameters
    ----------
    img : np.ndarray or nib.Nifti1Image or nib.Nifti2Image
        Numpy array or Nifti image. If array, Nifti image is created.
    path : os.PathLike or str
        Path to desired file.
    affine : np.ndarray, optional
        Affine matrix for the image, by default uses nibabel's default.
    kwargs : dict
        Additional arguments to pass to nib.save.

    Returns
    -------
    str
        Path of newly created file.
    """
    path = str(path)
    if not path.endswith(".nii") and not path.endswith(".nii.gz"):
        LOGGER.warning(
            "Nifti extension not detected in path! Niftis should end in .nii or .nii.gz! Appending .nii.gz..."  # pylint: disable=line-too-long
        )
        path += ".nii.gz"
    if isinstance(img, np.ndarray):
        img = nib.Nifti1Image(img, affine)
    nib.save(img, path, **kwargs)
    return path

wsiread(image_path)

Reads a Whole Slide Image from a file.

Allows operations on images larger than memory. wrapper for pyvips.Image.new_from_file()

Warnings

This function does not return a numpy array! You'll need to use proper WSI workflows (like tilewise operations) to do analyses on this! See the pyvips documentation for more information.

Parameters:

Name Type Description Default
image_path os.PathLike or string representing a file

Path to WSI.

required

Returns:

Type Description
Image

PyVips Image, see documentation for usage.

Source code in lavlab/imsuite.py
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def wsiread(image_path: Union[os.PathLike, str]) -> pv.Image:
    """Reads a Whole Slide Image from a file.

    Allows operations on images larger than memory.
    wrapper for pyvips.Image.new_from_file()

    Warnings
    --------
    This function does not return a numpy array!
    You'll need to use proper WSI workflows (like tilewise operations) to do analyses on this!
    See the pyvips documentation for more information.

    Parameters
    ----------
    image_path : os.PathLike or string representing a file
        Path to WSI.

    Returns
    -------
    pv.Image
        PyVips Image, see documentation for usage.
    """
    return pv.Image.new_from_file(str(image_path))

wsiwrite(img, path, use_fast_compression=None, **kwargs)

Writes an image to path. kwargs are passthrough to wrapped function.

Parameters:

Name Type Description Default
img Image

PyVips image.

required
path PathLike or str

Path to desired file.

required
use_fast_compression bool

Use fast compression as configured in context, by default uses bool from config.

None

Returns:

Type Description
str

Path of newly created file.

Source code in lavlab/imsuite.py
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def wsiwrite(
    img: pv.Image, path: Union[os.PathLike, str], use_fast_compression=None, **kwargs
) -> str:
    """Writes an image to path. kwargs are passthrough to wrapped function.

    Parameters
    ----------
    img : pv.Image
        PyVips image.
    path : os.PathLike or str
        Path to desired file.
    use_fast_compression : bool, optional
        Use fast compression as configured in context, by default uses bool from config.

    Returns
    -------
    str
        Path of newly created file.
    """
    path = str(path)
    if use_fast_compression is None:
        use_fast_compression = lavlab.ctx.histology.use_fast_compression
    if not kwargs:
        kwargs = (
            lavlab.ctx.histology.fast_compression_options
            if use_fast_compression
            else lavlab.ctx.histology.slow_compression_options
        )
    img.write_to_file(path, **kwargs)
    return path